Molecular modeling

Exploring protein conformations beyond fixed-end loops

A modular C++/Python sampler for protein loops, fragments, and intrinsically disordered regions, paired with explicit all-atom validation.

Ongoing research2026–Present

The question

How can a loop-closure method explore protein segments whose endpoints are free to move?

Fixed-end loop closure addresses an important but constrained sampling problem. Exploring fragments and disordered regions requires one or both endpoints to move while maintaining feasible local backbone geometry.

My work extends the geometric sampling machinery and separates conformational exploration from downstream evaluation of structural quality.

The approach

Flexible-endpoint sampling
Conceptual paths · not sampled structures · schematic
  1. 01

    Extend the sampler

    Support fixed or independently released endpoints, multiple loops, and rigid secondary-structure motion.

  2. 02

    Propose conformations

    Combine geometric endpoint moves with residue-specific Ramachandran priors and spatial-hash clash detection.

  3. 03

    Validate structures

    Reconstruct side chains, minimize with OpenMM, and assess conformational quality and ensemble diversity.

What I developed

  • Implemented released-anchor SE(3) proposals and multiple sampling frames.
  • Engineered spatial-hash collision detection and dense/sparse memory backends.
  • Integrated AttnPacker, OpenMM, and PhiSiCal-Checkup in a reproducible validation workflow.
  • Resolved stability and zero-output failure modes in the sampling software.
  • C++
  • Python
  • Protein sampling
  • OpenMM

Results & validation

  • Controlled comparisons with tested generative samplers showed broader exploration on the evaluated systems. This is a system-specific observation, not a claim of general superiority.
  • Ongoing work: no unpublished manuscript figures, raw benchmark data, or unfinished software releases are distributed here.

This project is ongoing. Comparisons are limited to the evaluated systems; no general superiority claim is implied.

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Research & collaboration

Let’s talk about the next question.

I’m interested in molecular-modeling methods, biomolecular simulation, and reusable scientific software.

sikaoguo@gmail.com